Reporter genes are commonly used in cell biology research. OZ Biosciences offers reporter mRNAs that can be used as controls to study transfection and expression in mammalian cells using a variety of assays. They mimic fully processed mature mRNA. These mRNAs are produced by in vitro transcription and stabilised at the 5’ end by modified nucleotides capping (Cap1). They contain a poly(A) tail at the 3’ end and are also optimized to yield improved stability and performance. Available as unmodified or modified versions: modified with 5-methoxyuridine (5moU replaces U) to reduce innate immune responses, modified with N1-methyl-pseudouridine (N1-mψ) to reduce innate immune response, or labelled with Cy5 so they can be easily traced to analyse mRNA delivery and translation efficiency.

GFP mRNA has been designed to produce high expression level of Green Fluorescent Protein. It is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509nm.

F-Luc mRNA has been designed to produce high expression level of FireFly Luciferase. It is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

Tomato mRNA has been designed to produce high expression level of Orange Fluorescent Protein. The detection can be done by fluorescent or confocal microscopy. The produced Tomato has an excitation maximum at 551-557nm and emission maximum at 579-583nm. Tomato expression level can also be monitored by fluorescence-activated cell sorter analysis (FACS).

mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in Discosoma sp. mCherry is a monomeric fluorophore with a peak absorption at 587nm and emission at 610nm. It is photostable and resistant to photobleaching.

β-galactosidase (β-gal) mRNA, codes for a protein product of the bacterial LacZ gene. β-gal catalyses the conversion of β-galactosides into monosaccharides. It is a common marker gene used to assess transfection efficiency.

R-Luc mRNA, have been designed to produce high expression level of Renilla Luciferase protein.

• No need of nuclear uptake - protein expression directly in cytoplasm
• Faster protein expression than DNA transfection
• No genomic integration
• Protein expression directly related to mRNA quantity
• Perfect for transfecting slowing or non-dividing cells
• Protein expression in a total promoter-independent manner
• Transient transfection: mRNA based expression of proteins sustains for a limited time
• Shipped on drug ice - storage at -80°C