Application

Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 2-8 µl Escort^™ III and 2 µg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. The following cells have been successfully transfected using Escort^™ III: A549C2C12 myotubesCardiomyocytes (rat)COS-7Fibroblasts (rat)Germ cells (male rat)Hepatocytes (rat and hamster)HepG2HeLaJurkatKeratinocytes (human)Myoblasts (mouse and quail)Myocytes (mouse)NIH3T3PC-12Retinal Neurons (rat)Tracheobronchial cells (sheep)

Caution

Do not freeze.

Components

Escort^™ III formulation:1 mg/mL total lipid in waterNote the identity of the lipids used in Escort^™ III is confidential.

Features and Benefits

Suitable for stable and transient transfection Optimized for a wide variety of primary cells Low toxicity Compatible with both serum and serum-free transfection protocols Ideal for PC-12 cells

General description

Escort^™ III is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into primary cells.

Legal Information

Escort is a trademark of Sigma-Aldrich Co. LLC

Principle

A stable complex is formed when Escort^™ III is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.