Application

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp. has been used in enzymatic conversion and derivatization of sulfatides. It has also been used to hydrolyze the fatty acid chain of mactosyl ceramide.

Biochem/physiol Actions

Hydrolyzes the N-acyl linkage between fatty acids and sphingosines in ceramides of various sphingolipids.

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp., under certain conditions, can recyclate lyso-sphingolipids. It condenses fatty acids to sphingosine to generate ceramide. The enzyme mainly acts on neutral and acidic glycosphingolipids.

General description

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp. is a hydrolytic enzyme.

Physical form

Solution in 50 mM sodium acetate, pH 6.0, with 0.1% Lubrol PX.

Unit Definition

One unit will hydrolyze 1 µmol of asialo GM₁ per minute at pH 6.0 at 37 °C.