Analysis Note

This strain binds less than 10% of the rabbit IgG bound by P7155 as assayed by a modification of the method of Kessler.

Application

Staphylococcus aureus has been used: to mimic infection and induce fever in Pekin duckto test its effect on hemocyte morphology in hemolymph samples from beetle Tenebrio molitor larvain the antibacterial activity and minimum inhibitory concentration (MIC) assay with gedunin and 7-deacetoxy-7αhydroxygedunin potassium salt

(Not intended for use as a starter culture.)

Wood 46, a non-protein A producing S. aureus strain, prepared by the same method as P7155 (Protein A, crude cell suspension-Cowan strain), may be used as a control in protein A-immunoglobulin binding studies.

Biochem/physiol Actions

Staphylococcus aureus Wood 46 displays reduced virulencecompared to the S.aureus. This isolate is useful in understanding protein A role in pathogenesis and virulence.

General description

Staphylococcus aureus Wood 46 is protein A deficientand spanegative. It shares 98% to 99% genome identity with S.aureus and shows a lower surface expression of cell wall-associated protein A.

Packaging

10, 50 mL in serum bottle

Physical form

Formalin-fixed crude cell suspension of essentially non-viable S. aureus (Wood 46 strain) in 0.05 M potassium phosphate buffer, pH 7.5, containing 0.2% sodium azide

Preparation Note

Produced in pure culture.