Analysis Note

C3 is depleted by immunoadsorption as judged by a highly sensitive hemolytic assay.

Application

Complement C3 deficient serum human has been used:as a component of the culture medium to study the influence of the inhibition of complement activation on complement receptors (CR3 and C3aR) expression in CD3+CD56+NKT-like cells as a negative control to evaluate the downstream complement activation post-mannan-binding lectin (MBL) pathway activationto study the opsonization of foreign particles by complement system

General description

Complement C3 deficient serum is a normal human serum in which C3 protein is removed by affinity chromatography. Depleted serum helps in the complement cascade up to the preferred convertase level. It eases the formation of enzyme complex from purified components.

Physical form

Supplied as a solution in phosphate buffered saline, pH 7.4