Application

Immunoprecipitation (IP) of FLAG- and 3xFLAG-tagged fusion proteins.Elution - FLAG peptide, Glycine, pH 3.5, 3x FLAG peptideBrowse additional application references in our FLAG^® Literature portal.

General description

EZ view Red Anti-FLAG M2 Affinity Gel is a resin that consists of Anti-FLAG M2 antibody, covalently bonded to 4% Agarose beads. The affinity gel is used to bind FLAG fusion proteins to samples, such as cell lysates and tissue, for purification of FLAG-tagged proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results. Agarose beads bind at N-terminal, Met-N-terminal and C-terminal FLAG fusion proteins, 3xFLAG-tagged fusion proteins.

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

Specificity

Suitable for purifying N-terminal, Met-N-terminal, C-terminal FLAG fusion proteins, 3xFLAG fusion proteins.