Application

S-protein Agarose has been used in the purification of recombinant S based tagged fusion proteins.

General description

It comprises a 15-amino acid sequence Lys-Glu-Thr-Ala-Ala-Ala-Lys-Phe-Glu-Arg-Gln-His-Met-Asp-Ser (KETAAAKFERQHMDS).

S-protein Agarose specifically retains S Tag™ fusion proteins. Binding can be performed in a column or batch mode. Elution is possible using slightly denaturing conditions or using a specific protease, such as thrombin or enterokinase, with appropriate recombinant proteins. The capacity of S-protein Agarose varies and is based on the size and folding characteristics of a given target protein.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

10, 2 ml in Plastic ampoule

Warning

Toxicity: Standard Handling (A)