Application

Single day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using magnetic A beads.

Components

Magnetic Protein A Beads ChIP Dilution Buffer Low Salt Wash Buffer High Salt Wash Buffer LiCl Wash Buffer TE Buffer Cell Lysis Buffer Nuclear Lysis Buffer ChIP Elution Buffer (w/o Proteinase K) 10X Glycine 10X PBS Protease Inhibitor Cocktail II Proteinase K Spin Filters Collection Tubes Bind Reagent A Wash Reagent B Elution Reagent C

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Faster:Magnetic protein A beads allow for the entire ChIP protocol to be done in as little as a day! All reagents to process your samples are included - you don′t have to spend valuable time making them. Easier: Spin columns make DNA purification easier and more reliable - no more messy phenol-chloroform extractions.

General description

Chromatin Immunoprecipitation (ChIP) is an important technique allowing the researcher to analyze in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.

Legal Information

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

Two boxes containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).

Storage and Stability

Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.