Application

Research CategoryEpigenetics & Nuclear Function

Used to detect/quantify: Protein A+G

Components

Magna ChIP protein A/G magnetic beadsHT96 Nuclei Isolation Buffer HT96 ChIP Buffer (Sonication/ChIP/Wash)Low Stringency IP Wash Buffer HT96 ChIP Elution Buffer Proteinase K SolutionProtease Inhibitor Cocktail III10X Glycine 10X PBS 96 Well ChIP Plate 96 Well Thermal PlatePlate SealStrip Caps

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format. This reliable streamlined approach works for both cells and tissues and can be performed using either a multi-channel pipette or an automated liquid handling instrument.Each kit includes a complete set of validated, quality controlled reagents, plus a detailed protocol. Features & Benefits Complete set of materials for up to 96 ChIP reactions Protein A+G bead blend for ChIP with a broader range of antibodies than A or G alone Low Chromatin requirements: 10, 000 to 100, 000 cells per reaction Optimized streamlined protocol with only a single buffer for sonication, IP, or wash; and protocols for automated liquid handling systems Protocols for using cells or tissues Direct analysis of DNA without additional clean-up steps Compatible with ChIPAb+ validated antibody and primer sets

Legal Information

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

Kit capacity: 96 chromatin immunoprecipitation assays

Physical form

Two boxes containing all necessary reagents to perform 96 chromatin immunoprecipitation reactions in a 96 well plate format. Supplied buffers are sufficient to generate 24 individual chromatin preparation reactions using 15 cm plates of cultured cells or tissue samples.

Storage and Stability

Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.