Poly(ethylene glycol), BioUltra, for molecular biology, 1,000

Code: 81189-250G D2-231

Application

Poly(ethylene glycol) has been used to develop a method for efficient transformation of Brevibacterium lactofermentum protoplasts with plasmid DNA. It ha...


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Application

Poly(ethylene glycol) has been used to develop a method for efficient transformation of Brevibacterium lactofermentum protoplasts with plasmid DNA. It has been used as a fusion agent to fuse spleen cells with the myeloma cell line SP2/0.

General description

Poly(ethylene glycol) (PEG) is a non-ionic hydrophilic polymer and is available in different molecular weights. It helps in the purification and crystal growth of proteins and nucleic acids. PEG and dextran together result in aqueous polymer two phase system, which is required for the purification of biological materials. PEG also interacts with cell membrane, thereby allowing cell fusion.

Other Notes

PEG precipitation of DNA fragments

λ50 mg/mL in H2O
anion tracessulfate (SO42-): ≤50 mg/kg, chloride (Cl-): ≤50 mg/kg
cation tracesBi: ≤5 mg/kg, Mo: ≤5 mg/kg, Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Li: ≤5 mg/kg, K: ≤200 mg/kg, Na: ≤200 mg/kg, Cr: ≤5 mg/kg, Mn: ≤5 mg/kg, Sr: ≤5 mg/kg, Mg: ≤5 mg/kg, Ca: ≤10 mg/kg, Pb: ≤5 mg/kg, Cu: ≤5 mg/kg, Ba: ≤5 mg/kg, Fe: ≤5 mg/kg, Zn: ≤5 mg/kg, Ni: ≤5 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg
formsolid
gradefor molecular biology
impuritiesphosphatases, none detected, RNases, none detected, proteases, none detected, insoluble matter, passes filter test, DNases, none detected
InChI keyLYCAIKOWRPUZTN-UHFFFAOYSA-N
InChI1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
mol wtMr 950-1050
pH5.5-7.0 (25 °C, 50 mg/mL in H2O)
product lineBioUltra
Quality Level200, 100
SMILES stringC(CO)O
solubilityH2O: 50 mg/mL at 20 °C, clear, colorless
UV absorptionλ: 260 nm Amax: ≤0.10, λ: 280 nm Amax: ≤0.05
Code
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Unit Size
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81189-100G
Poly(ethylene glycol), BioUltra, for molecular biology, 1,000

Unit:100G
List Price: £43.10
Source:List Price
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81189-1KG
Poly(ethylene glycol), BioUltra, for molecular biology, 1,000

Unit:1KG
List Price: £209.00
Source:List Price
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Cas Number25322-68-3
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