Lyophilised positive control primers/probe (150 reactions), positive control RNA template, RNAse/DNAse free water.

Negative test results are only valid when the suitability of the reagents, template and protocols have also been demonstrated. The Primerdesign real-time PCR internal control kit contains control RNA. This can be spiked in to your sample prior to nucleic acid extraction or diluted and spiked directly into your real-time PCR reaction. A positive signal of around Cq 25 indicates that your experimental procedure is working well.

The internal control kit can be multiplexed, or used in parallel with your test samples. The assay is primer limited and based on an obscure nucleic acid sequence so will not therefore interfere with the detection of your target sequence.

• Perfect Internal control
• Taqman style probe
• Available to read through VIC channel.
• Primer limited to prevent interference with target gene primers