Application

β-Nicotinamide adenine dinucleotide hydrate has been used:to measure adenosine triphosphate (ATP) synthesis rates (kinetics measurements)in the reaction buffer for pyruvate kinase (PK) activity assayas a component of master mix for a high throughput screening

Biochem/physiol Actions

β-Nicotinamide adenine dinucleotide (NAD) cycles between the oxidized (NAD⁺⁾ and reduced (NADH) forms to maintain a redox balance necessary for continued cell growth. It significantly participates in enzyme-catalyzed oxido-reduction processes and many genetic processes. It is also involved in microbial catabolism.

Electron acceptor

General description

β-Nicotinamide adenine dinucleotide (NAD) is an electron carrier and a cofactor. It is ubiquitously found in the living system. NAD⁺ contains an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. In NAD⁺ complexes, the enzyme-cofactor interactions are highly conserved.

Other Notes

This is the common form of NAD.

Crystalline NAD contains less than 0.1% (w/w) residual organic solvent, is less hygroscopic and electrostatic, and is more stable at ambient temperatures than amorphous NAD.

Packaging

Packaged by solid weight.

1 g in poly bottle

25, 100, 250 mg in poly bottle