HiTrap Phenyl FF (Low Sub) are prepacked Phenyl Sepharose 6 Fast Flow columns for hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elution conditions.

• Provide convenient and fast small-scale protein purifications
• Phenyl Sepharose 6 Fast Flow is a well-established, standard aromatic HIC resin for capture and intermediate purification requiring low to resin hydrophobicity
• Two levels of substitution on Phenyl Sepharose 6 Fast Flow help find optimal selectivity and binding capacity
• Flexible design for use with syringes, single laboratory pump, or chromatography system such as ÄKTA design
• High flow rates and high capacities
• Excellent and easy scale-up achieved by connecting several HiTrap columns in series or using HiPrep columns