Application

UHPLC Analysis of Peptides on BIOshell^™ A160 Peptide C18, CN, and Phenyl-Hexyl, 2.7 µm

General description

BIOshell^™ 160Å Peptide CN columns provide an alternative selectivity to the traditional C18 bonded phases. The introduction of the cyano group enables peptides and small proteins to be separated due to a combination of hydrophobicity and dipole-dipole interactions making this column an excellent choice for biomolecules that are not resolved on traditional C18 columns. These columns contain advanced Fused-Core particles with pores strategically sized to 160 Å to enable optimized peptide diffusion. This attribute makes these columns an excellent choice for peptide mapping. Additionally, the sterically-protected cyanopropyl ligands provide extra stability allowing the columns to be used at an extended pH range (2-9) and high temperatures (up to 90 °C).

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

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