Analysis Note

The target analytes include acids, bases, and polar analytes.

Application

HPLC Analysis of Water Soluble Vitamins on Ascentis^® Express AQ-C18, 5 µm

General description

Ascentis^® Express AQ-C18 is a high-speed, high-performance liquid chromatography column based on the Fused-Core^® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.6 µm thick porous shell and the small overall particle size of 5 µm. The AQ-C18 is a C18 bonded phase prepared using a proprietary procedure that incorporates a small amount of polar silane which makes the phase resistant to dewetting. This resistance to dewetting allows the users of the AQ-C18 phase to run highly-aqueous (up to 100%) mobile phases. The modified C18 phase exhibits similar retention to Ascentis^® Express C18 with a different selectivity, adding a valuable alternative for resolving difficult separations. The AQ-C18 is a reversed-phase packing that can be used for basic, acidic, and neutral compounds.

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.