Analysis Note

Target analytes include: monoclonal antibodies, antibody-drug conjugates, antibody fragments and large proteins with molecular weights up to 500 kDa.

Application

UHPLC Analysis of Reduced IgG1 on BIOshell^™ IgG 1000 Å DiphenylUHPLC Analysis of IgG2 on BIOshell^™ IgG 1000 Å C4, C18, and Diphenyl, 2.7 µm

General description

BIOshell^™ IgG 1000 Å Diphenyl is a high-speed, high-performance liquid chromatography column based on a new wide-pore (1000 Å) Fused-Core^® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5 µm thin porous shell and the small overall particle size of 2.7 µm. The densely bonded, extensively endcapped diphenylmethyl bonded phase of BIOshell^™ IgG 1000 Å Diphenyl provides a stable, reversed-phase packing that can be used for separating high molecular weight compounds, such as proteins.

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.