Discovery BIO Wide Pore C8 HPLC Column, 10 mum particle size, L x I.D. 25 cm x 21.2 mm

Code: 567225-U D2-231

General description

Discovery BIO Wide Pore C8 exhibits hydrophobicity intermediate between the Discovery BIO Wide Pore C5 and the Discovery BIO Wide Pore C18. The difference...


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General description

Discovery BIO Wide Pore C8 exhibits hydrophobicity intermediate between the Discovery BIO Wide Pore C5 and the Discovery BIO Wide Pore C18. The difference in hydrophobicity gives it unique selectivity relative to these other phases. It is ideal for peptide mapping because it provides complementary information compared to a C18 separation. Because of its intermediate hydrophobicity, we also recommend it for method development or scouting work. As with all Discovery BIO Wide Pore phases, the C8 phase gives efficient, symmetrical peaks, exceptional stability, long column lifetime, and LC-MS compatibility.

Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC

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agencysuitable for USP L7
extent of labeling5% Carbon loading
featureendcapped
L × I.D.25 cm × 21.2 mm
manufacturer/tradenameDiscovery®
materialstainless steel column
matrix active groupC8 (octyl) phase
matrixfully porous particle, silica particle platform
operating pH2-8
packaging1 ea of
parameter400 bar pressure (5801 psi), 0-70 °C temperature
particle size10 µm
pore size300 Å
product lineDiscovery®
Quality Level100
separation techniquereversed phase
surface area100 m2/g
surface coverage4 µmol/m2
technique(s)HPLC: suitable, LC/MS: suitable
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