General description

Ascentis Express 5 µm OH5 is a high-speed, high-performance liquid chromatography column based on a 5-micron Fused-Core^® particle design. The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shorter diffusion paths in the 0.6-micron thick porous shell and an overall particle size of ~5-microns. The Ascentis Express 5 µm OH5 stationary phase is a highly polar ligand that possesses 5 hydroxyl groups tethered to the silica via novel proprietary linkage chemistry. This high performance material provides a column that can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, zwitterionic, or neutral compounds. Column CharacteristicsThe Fused-Core particle has a surface area of ~ 100 m²/g and an average pore size of 90Å. The Fused-Core particles are 30% to 50% heavier than commercially available totally porous particles due to the density of the solid cores. Therefore, the effective surface area per column is similar to columns packed with totally porous particles having surface areas in the 160-200 m²/g range.

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis