Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay and in DNA-binding assay.

Features and Benefits

Activated No nuclease activity detected after 16 h at 37 °C incubation High quality template DNA

General description

High quality single-stranded template DNA isolated from the thymus of male and female calves.

Preparation Note

Prepared by a modification of the method of Alberts and Herrick from calf thymus DNA.

Quantity

One mg DNA is approx. 25 A₂₆₀ units.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.