Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay. It was used as reference to determine the DNA content in cartilage tissue digest samples.

Features and Benefits

No nuclease activity detected after 16 h at 37 °C incubation High quality template DNA

General description

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Packaging

5 units in glass bottle

0.25, 1 unit in glass bottle

Preparation Note

Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of ~2 A₂₆₀ units per mL. ~20 A₂₆₀ units per mg DNA.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Specificity

42% GC; T_m = 87 °C.% GC is the percentage of G/C base pairs. T_m is the temperature at the midpoint of the thermal denaturation profile.