Nucleon PhytoPure produces the DNA quality, length, and purity of a phenol-chloroform extraction without phenol.
- Efficiently removes plant polysaccharides ensuring high yields of high-quality plant genomic DNA in only 1 h
- Two kit sizes provide economical scale-up using the same phenol-free protocol
Nucleon PhytoPure, a DNA extraction kit for high-quality plant and fungal genomic DNA purification
Nucleon PhytoPure extracts genomic DNA from plant and fungal samples in a fraction of the time taken by conventional methods, while efficiently removing proteins and polysaccharides. Polysaccharides are very common contaminants in plant DNA extracts, and often result in difficult-to-handle, "slimy" DNA pellets. Although most plant DNA extraction techniques are effective at removing proteins, they are much less successful with polysaccharides.
Nucleon PhytoPure proprietary resin is added following cell lysis with Potassium SDS, and a single chloroform extraction. Aspiration of the top layer of nucleic acids is simplified and the volume of usable sample maximized by a barrier formed between the two layers. The combination of purity and high molecular weight makes the recovered DNA suitable in multiple downstream molecular biology applications, including whole genome amplification (WGA) for applications such as next generation sequencing.
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