Analysis Note

E.coli forms blue colonies. Other coliforms will show as red colonies. All other colors are non-coliform bacteria.

Target Microorganisms: Total coliforms and E. Coli

QC organismsE. cloacae (ATCC 23355)E. coli (ATCC 25922)K. pneumoniae (ATCC 13883)P. aeruginosa (ATCC 27853)

Application

m-Coliblue24 broth is used for monitoring drinking water and wastewater using a 24-hour incubation period. It can also be used to detect coliforms in other types of water—bottled, surface, ground, well, recreational (fresh or marine), as well as process water for ultrapure chemical processing.

Culture Medium

m-ColiBlue

General description

Compatible with Absorbent Pads EO sterilized items AP10047E0 or Milliflex™ Liquid Media Cassette. Do not use Gamma sterilized Millipore items.

m-ColiBlue24 Broth allows for the simultaneous detection of total coliform bacteria and Escherichia coli (E. coli) within 24 hours. An enzymatic indicator in the medium causes non-fecal total coliform colonies grown on the m-ColiBlue24 medium to be red, while the E. coli (fecal coliform) colonies are blue.{26} The selectivity of the enzymatic indicator eliminates the need for confirmation. The low false positive and false negative rates allow for the detection of at least 95% of all E. coli. M-ColiBlue24 Broth enhances the growth rate of coliform bacteria. Special inhibitors in the medium efficiently minimize the growth of non-coliform bacteria.{27}

Legal Information

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

50×2 mL in ampoules

2 mL plastic ampoules

Specifications

Incubation Temperature: 35 °C ± 0.5

EPA Approved

Incubation Time: 24 hrs