Application

Sulfatase from Aerobacter aerogenes has been used:as a deconjugation enzyme for treating plasma samples for quercetin quantification using liquid chromatography with tandem mass spectrometry (LC/MS/MS) analysesin fluorescence intensity-based enzymatic assay with an activity-based probe probe 1to treat sulfatide liposomes to remove the 3-O-sulfogalactosyde head from sulfatides

Biochem/physiol Actions

Sulfatases hydrolyze sulfate ester bonds to generate inorganic sulfates. Microbial sulfatases participate in sulfur scavenging and are essential enzymes for the utilization of sulfur. They may be associated with pathogenesis. Commercially available sulfatase from Aerobacter aerogenes is useful as a deconjugation enzyme for the removal of glucuronate and sulfate moieties from conjugates. Sulfatases find application in industry and agriculture.

General description

Sulfatases comprise Cys/Ser-X-Pro-X-Arg motif that is conserved in their active sites.

Physical form

Solution in 50% glycerol containing 0.01 M Tris, pH 7.5.

Unit Definition

One unit will hydrolyze 1.0 µmole of p-nitrophenyl sulfate per min at pH 7.1 at 37 °C.