Application

SIGMAFAST^™ DAB with Metal Enhancer has been used in the visualization of lung tissue sections using immunohistochemistry, dorsal root ganglion (DRG) neurons and immunohistochemical staining for collagen in anterior cruciate ligament (ACL) cells.

The DAB reaction is enhanced by the addition of CoCl₂ and produces a more intense stain which is resistant to alcohol.

General description

SIGMA FAST^™ DAB with Metal Enhancer Tablets require no additional buffers or steps to prepare an active substrate solution for detection of peroxidase activity. Commom use is in immunohistology as a precipitating substrate.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Reconstitution

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB with a metal enhancer.