Compared to similar stains, the more finely controlled colloidal structure of ProtoBlue Safe improves both the sensitivity and the universality of staining. ProtoBlue Safe is less prone to high background caused by trace residual SDS in the gel.

Laboratories typically spend twice as much per gel in the methanol and acetic acid for staining and destaining with regular Coomassie than for ProtoBlue Safe (including ethanol cost). In addition, ProtoBlue Safe is much faster, more sensitive, and you can pour the used stain solution down the drain. Protein visualization is faster, safer, more sensitive and less expensive with ProtoBlue Safe.

Sample can be detected in three easy steps:

1. Wash gel three times for five minutes with deionized water on an orbital shaker. (Note: This step is optional. A longer incubation in stain solution and longer post-stain washing can substitute for this wash step.)

2. Add one part ethanol to nine parts staining solution while stirring. Adding ethanol greatly improves sensitivity, reproductivity and shelf life compared to solutions that add ethanol at point of manufacture.

3. Add enough staining solution to completely cover the gel. Bands containing more than 1 µg will be detected within 15 minutes. (Note: For sensitivity up to 5 ng, incubate the gel in stain for at least five hours.)

Keep in a tightly closed container, stored in a cooled, dry, ventilated area. Avoid contact and inhalation. Do not get in eyes, on skin, on clothing. Wash thoroughly after handling.

• Fast, easy to use protocol
• Sample can be detected with three quick, easy steps
• Eco-friendly; non-hazardous disposal
• Costs less than regular Coomassie
• Long shelf life - stable for two years stored at room temperature in a cool, dry place