Analysis Note

Tested for use in denatured polyacrylamide gel electrophoresis.

Application

Sodium dodecyl sulfate has been used: in chromatin immunoprecipitation in SDS-polyacrylamide gel electrophoresis (SDS-PAGE) as a component of radioimmunoprecipitation assay buffer for immunoblotting

Used to solubilize and denature proteins for denaturing-PAGE. Most proteins bind SDS in a ratio of 1.4 g SDS per gram of protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on their size.

Anionic detergent

General description

Sodium dodecyl sulfate (SDS) is an anionic detergent suitable to denature proteins. It has a long-chain aliphatic group with negatively charged sulfate attached. This makes SDS an amphipathic detergent.

Packaging

25, 100, 500 g in poly bottle

1 kg in poly bottle