Application

Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.

Components

1x MESA buffer contains: 40 mM MOPS 10 mM sodium acetate 1 mM EDTA (pH 8.3)

General description

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.

Packaging

1 L in poly bottle

Reconstitution

Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.