Application

Suitable for size determination of small RNA using native or denaturing agarose gel electrophoresis. Transcript RNA Markers 0.2-10 kb has been used as a molecular weight marker for northern blot hybridization.

Components

Sigma’;s Transcript RNA Marker (0.2 – 10 kb) is provided in a gel-loading solution with 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

General description

Sigma’;s Transcript RNA Marker (0.2 – 10 kb) contains 9 RNA transcripts from 200 – 10,000 bases. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Other Notes

Contains nine defined RNA transcripts, 200–10,000 nucleotides

For optimal resolution, the recommended agarose gel concentration is 1.0%.