The newest addition to the SequaGel family, the UreaGel 29:1 System allows researchers to easily and safely produce denaturing gels of the popular 29:1 acrylamide: bis-acrylamide formula in concentrations up to 20% monomer. These gels are ideal for analysis of DNA or RNA.
The UreaGel 29:1 System consists of UreaGel 29:1 Concentrate, UreaGel Buffer and UreaGel Diluent. UreaGel 29:1 Concentrate contains 241.7 g acrylamide, 8.3 g methylene bisacrylamide per litre in 7.5M urea in a deionized aqueous solution. UreaGel Diluent consists of a deionized aqueous solution of 7.5M urea. UreaGel Buffer contains 0.89M Tris-Borate, 20mM EDTA and 7.5M urea at pH 8.3.
Storage: UreaGel 29:1 Concentrate, UreaGel Diluent, and UreaGel Buffer are stable for one year when stored tightly capped, in a dark area at room temperature. Urea may precipitate if these solutions are refrigerated. This urea will redissolve when the solution returns to room temperature.
- Casts 29:1 denaturing gels for RNA or DNA analysis
- Easily cast up to 20% gels
- Certified nuclease free
- Consistently crystal clear gels
- Requires addition of TEMED and ammonium perfsulfate prior to casting
This product has met the following criteria: