Agarose, Type I, low EEO

Code: A6013-5G D2-231

Analysis Note

The following is a list of properties associated with our agaroses:Sulfate content - used as an indicator of purity, since sulfate is the major...


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£33.70 5G
£40.44 inc. VAT

Analysis Note

The following is a list of properties associated with our agaroses:Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.Gel strength - the force that must be applied to a gel to cause it to fracture.Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature. Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Application

Agarose has been used:in the embedding of fluorescent nanospheres.for the generation of tissue-like phantoms similar to myocardial tissues.in hydrogel preparation.

General description

Agarose is a polymer of agarobiose (L- and D-galactose) subunits. It is isolated from the seaweed Gelidium and Gracilaria. Agarose forms non-covalent network of bundles. It is exploited for the separation of nucleic acids. The concentration and pore size of the gel are inversely related. Agarose has wide range of separation and is simple and rapid to prepare without the need of catalyst.

Packaging

1 kg in poly bottle

5, 10, 25, 50, 100, 250, 500 g in poly bottle

anion tracessulfate (SO42-): ≤0.15%
biological sourcealgae (Gelidium & Gracilaria)
EEO0.09-0.13
formpowder
gel strength≥1200 g/cm2 (1% gel)
impurities≤10% water
InChI keyMJQHZNBUODTQTK-WKGBVCLCSA-N
InChI1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
Quality Level200
technique(s)electrophoresis: suitable
transition tempgel point 36 °C ±1.5 °C (1.5% gel)
typeType I
Code
Description
Unit Size
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A6013-100G
Unit:100G
List Price: £273.00
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A6013-25G
Unit:25G
List Price: £104.00
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A6013-10G
Unit:10G
List Price: £52.70
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A6013-1KG
Unit:1KG
List Price: £1,970.00
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A6013-250G
Unit:250G
List Price: £648.00
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A6013-500G
Unit:500G
List Price: £1,100.00
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A6013-50G
Unit:50G
List Price: £195.00
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Cas Number9012-36-6
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