Application

Research Sub CategoryLive Cell Dye

Fluorescent protein gel stain that requires only a single 5-30 minute staining step without fixation.

Protein gel stain

Research CategoryCell Culture

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

EZFluor^™ UV 1-step Fluorescent Protein Gel Stain is a ready-to-use luminescent protein gel stain designed for imaging using a UV transilluminator. The EZFluor™ UV protein stain requires only a single 5-30 minute staining step without fixation. Destaining is optional. Moreover, the EZFluor™ UV protein stain offers safer handling and ease of disposal, because it is an aqueous-based solution that does not contain hazardous methanol or acetic acid. The EZFluor UV protein stain (after pH neutralization) passed environmental toxicity testing and is classified as non-hazardous to the environment under CCR Title 22 regulations.

EZFluor™ UV 1-step Fluorescent Protein Gel Stain can detect as little as 1-10 ng of protein per band depending on the staining method used. The results obtained with EZFluor UV protein stain are comparable to those with Oriole™ Fluorescent Gel Stain; however, the EZFluor UV protein stain has a more convenient protocol, does not contain hazardous solvents, and does not cause gel shrinkage. Staining is fully compatible with mass spectrometry and Edman-based sequencing.

Spectral Properties
Absorbance: 288 nm
Emission: 603 nm

Legal Information

EZFluor is a trademark of Sigma-Aldrich Co. LLC

Physical form

Liquid

Quality

Spectral Properties
Absorbance: 288 nm
Emission: 603 nm

Storage and Stability

Store EZFluor^™ UV 1-step Fluorescent Protein Gel Stain at 2-8°C. Protect From Light.