Application

Nucleic acid detection

Research Sub CategoryLive Cell Dye

Research CategoryCell Culture

GelGreen™ is a fluorescent nucleic acid stain designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

GelGreen™ is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye specifically designed for gel staining. GelGreen™ has UV absorption between 250 nm and 300 nm and a strong absorption peak centered around 500 nm (Figure 1). Thus, GelGreen™ is compatible with either a 254 nm UV transilluminator or a gel reader equipped with visible light excitation (such as a 488 nm laser-based gel scanner or a Dark Reader). GelGreen™ is far more sensitive than SYBR™ Safe (Figure 2). Unlike SYBR™ dyes, which are known to be unstable, GelGreen™ is very stable, both hydrolytically and thermally.

The dye is noncytotoxic and non-mutagenic at concentrations well above the working concentrations used in gel staining. GelGreen™ successfully passed environmental safety tests in compliance with CCR Title 22 Hazardous Waste Characterization, under which GelGreen™ is not classified as hazardous waste.

Spectral Properties
Absorbance: Standard Transilluminator (254 nm) such as a Dark Reader™
Emission: Long path green filter such as a SYBR™ filter or GelStar™ filter.

Legal Information

GelGreen is a registered trademark of Biotium Inc.

Physical form

Liquid

Quality

Spectral Properties
Absorbance: Standard Transilluminator (254 nm) such as a Dark Reader™
Emission: Long path green filter such as a SYBR™ filter or GelStar™ filter.

Storage and Stability

GelGreen™ is a very stable dye. Store GelGreen™ at room temperature, protected from light. Dye precipitation may occur at lower temperatures, resulting in lower signal or the appearance of precipitate on the surface of the gel. If this occurs, heat the solution to 45-50°C for two minutes and vortex. Protect From Light.