General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Reconstitution

Designed for use in stable isotope labeling with amino acids in cell culture applications.Supplement with 0.100 g/L L-Arginine-¹³C₆, ¹⁵N₄ HCl (Product 608033), 0.100 g/L L-Leucine-¹³C₆, ¹⁵N (Product 608068), and 0.100 g/L L-Lysine-¹³C₆, ¹⁵N₂ (Product 608041).