ECF Substrate

Code: RPN5785 D2-84

Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560nm when excited at the appropriate wa...


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£228.00 EACH
£273.60 inc. VAT

Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560nm when excited at the appropriate wavelength.

  • A fluorescent substrate for alkaline phosphatase-based detection of protein blots, for use with fluorescence scanning equipment such as Storm instruments
  • For use in western blotting, dot, and slot blotting applications
  • Sensitivity comparable to chemiluminescence
  • The wider dynamic range of fluorescence scanning systems compared with film allows visualisation and quantitation of weak and dark bands in the same scan
  • Eliminates need for darkroom and film
  • The following reagents are provided, sufficient for detecting 2500cm2 membrane: 36mg ECF substrate and 60mL dilution buffer

PackFor 2500cm2 membrane
This product has met the following criteria: