The ToxiLight™ 100% Lysis Reagent set is intended for use with the ToxiLight™ Non-destructive Cytotoxicity Assay (sold separately) and is designed to give the user a 100% lysis or total adenylate kinase control. It contains the 100% Lysis Reagent, 10mL and Tris-Acetate Buffer, 50mL (required volume correction for sample wells), and is suitable for 200 tests in a 96 well format.

Simply add the 100% Lysis Reagent to the control wells at a ratio of 1:2 (lysis reagent : sample volume), volume correct the remaining sample wells with Tris-Acetate Buffer and then incubate at room temperature for 10 minutes.

The ToxiLight™ 100% Lysis Reagent should be included as a control at each time point within your cytotoxicity assay. After addition proceed with the standard ToxiLight™ Assay protocol.

• Highly sensitive - the ToxiLight™ BioAssay Kit exploits the cyclic nature of the AK reaction whereby a small amount of the AK enzyme can generate high concentrations of ATP. This enables the detection of very subtle changes in cytotoxicity and reduces false negatives
• Non-destructive - ToxiLight™ assay detects cellular AK present in cell culture supernatants, eliminating the need to lyse cells to perform the assay, and allowing multiple tests to be performed on the same sample
• Simple - assay requires the simple addition of a single reagent, which can be added directly to wells in which cells are growing, or to a small sample of aspirated culture supernatant
• Fast - readable signal is detectable 5 minutes after the addition of the ToxiLight™ BioAssay reagent
• Flexible - cell supernatants can be frozen with no loss of AK activity, allowing for long-term kinetic studies
• Safe - no hazardous chemicals are required