Amersham ECL Plex Cy3 and Cy5 labelled antibodies are part of an optimised system for quantitative analysis using fluorescent Western blotting. Fluorescence detection is a direct Western blotting method in which a secondary antibody is conjugated to a fluorophore, avoiding the need for ancillary detection reagents.
Amersham Cy3 and Cy5 secondary antibodies are labelled with near-infrared (NIR) fluorophores that emit light at wavelengths of 570 and 670nm. By using Amersham Cy3 and Cy5 secondary antibodies together with a pair of appropriate rabbit or mouse primary antibodies, you can perform multiplexed experiments.
- Broad dynamic range
- High sensitivity
- Ability to detect multiple proteins on same blot (multiplexing)
- Wide pH tolerance (3 to 10), making them compatible with most Western blotting buffers
- High photostability
- Signal longevity >3 months
This product has met the following criteria: