Analysis Note

O-Phospho-L-tyrosine conjugated to bovine serum albumin

Application

Phosphotyrosine-BSA may be used for specific inhibition of phosphotyrosine in platelet extracts, in immunoblotting and ELISA applications, at a working concentration of 5 µg/mL.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The activity of proteins depends on phosphorylation of specific amino acid residues. In applications involving identification of activated cellular proteins, it is important to confirm specificity of antibodies used against a particular phosphorylated amino acid. For such applications, inhibitors of phosphorylated antibodies on specific amino acid residues are suitable. Phosphotyrosine antibody inhibitor may be used for the specific inhibition of the reactivity of anti-phosphotyrosine antibodies but not that of anti-phosphothreonine- or anti-phosphoserine-specific antibodies.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Phosphotyrosine antibody inhibitor that specifically blocks the reactivity of anti-phosphotyrosine antibody. It does not block the activity of anti-phosphothreonine or anti-phosphoserine-specific antibodies.