Analysis Note

Positive ControlNIH3T3 cells

Application

ELISA (0.1 g/ml)Flow Cytometry (1 g/ml)Frozen Sections (5-10 g/ml) Immunoblotting (5 g/ml)Immunofluorescence (1 g/ml)Paraffin Sections (5-10 g/ml)Radioimmunoassay (0.1 g/ml)Southwestern Blot (5 g/ml)

General description

This Anti-5-Methylcytosine Mouse mAb (162 33 D3) is validated for use in ELISA, FC, Frozen Sections, IF, Paraffin Sections, Radioimmunoassay, Southwestern Blot for the detection of 5-Methylcytosine.

Recognizes 5-methylcytosine in methylated DNA or RNA in NIH3T3 cells.Specific Affinity purified mouse monoclonal antibody - Detects methylated DNA from a broad range of species. Several Applications Flow Cytometry Frozen Sections Immunoblotting Immunofluorescence Paraffin Sections r>Reliable Generates reproducible results. Clone 162 33 D3 has been used in over 50 publications.

Immunoaffinity purified mouse monoclonal antibody. Recognizes 5-methylcytosine.

Immunogen

5-methylcytosine conjugated to ovalbumin

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Ehrlich, M. 2002. Oncogene21, 5400. (Review)Widschwendter, M. and Jones, P.A. 2002. Oncogene21, 5462. (Review)Barton, S.C., et al. 2001. Hum. Mol. Genet.10, 2983.Piyathilake, C.J., et al. 2001. Hum. Pathol.32, 856.Taddei, A., et al. 2001. Nat. Cell Biol.3, 114.Piyathilake, C.J., et al. 2000. Biotech. Histochem.75, 251.De Capoa, A., et al. 1999. FASEB J.13, 89.

Clone 162 33 D3 is useful for the quantitative and qualitative detection of methylated DNA or RNA in a variety of samples and applications. It has been used in over 40 publications. Antibody should be titrated for optimal results in an individual systems.

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)