Analysis Note

Negative ControlU2OS cells

Positive ControlSaos-2 cells or bladder tissue

Application

Immunoblotting (0.5-5 µg/ml, see application references)

General description

This Anti-p16 (Ab-1) Mouse mAb (DCS-50.1/H4) is validated for use in Immunoblotting for the detection of p16 (Ab-1).

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-2 mouse myeloma cells. Recognizes the ~16 kDa p16 protein.

Recognizes the ~16 kDa p16 protein in Saos-2 cells and bladder tissue. Sold under license of U.S. Patent 6,482,929. Sold under license of U.S. Patent 5,843,756, 6,090,578, and corresponding patents.

Immunogen

recombinant, human p16

Human

Legal Information

Sold under license of U.S. Patents 5,843,756, 6,090,578 and corresponding patents.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Koh, J., et al. 1995. Nature375, 506.Lukas, J., et al. 1995. Nature375, 503.Cheng, J.Q., et al. 1994. Cancer Res.54, 5547.Jen, J., et al. 1994. Cancer Res.54, 6353.Kamb, A., et al. 1994 Science264, 436.Nobori, T., Miura, K., et al. 1994. Nature368, 753.Okamoto, A., Demetrick, D.J., et al. 1994. Proc. Natl. Acad. Sci. USA91, 11045.Serrano, M., et al. 1993. Nature366 704.

Does not cross-react with the closely related p15 protein. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin pH 7.5.

Warning

Toxicity: Standard Handling (A)