Analysis Note

ControlDuctal carcinoma tissue

Application

Anti-Nectin-4/PVRL4 Antibody, clone N4.61 is an antibody against Nectin-4/PVRL4 for use in IH, ELISA & IP.

Research CategoryCell Structure

ELISA Anaysis: A previous lot was used by an independent laboratory in ELISA (Fabre-Lafay, S., et al. (2005). Journal of Biological Chemistry. 280(20):19543–19550).

Immunoprecipitation Anaysis: A previous lot was used by an independent laboratory in IP (Fabre-Lafay, S., et al. (2005). Journal of Biological Chemistry. 280(20):19543–19550).

Research Sub CategoryECM Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nectin-4 is also known as poliovirus receptor-related protein 4 (PRR4 or PVRL4). Nectins are known for being the receptors for the herpes simplex virus. Nectin-4 is an immunoglobulin-like transmembrane cell adhesion protein that also seems to play a role in neural tube formation, with N-cadherin. Nectin-4 is connected with the actin cytoskeleton through afadin inside the cell. Nectin-4 is predominantly found in human placenta although in mice, expression is wide-ranging. Nectin-4 seems to adhere primarily to cells expressing nectin-1 and vice versa.

Immunogen

Recombinant protein corresponding to human Nectin-4.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG1κ Supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Immunohistochemistry in ductal carcinoma tissue.

Immunohistochemistry Analysis: A 1:400 dilution of this antibody detected Nectin-4/PVRL4 in ductal carcinoma tissue.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

55 kDa calculated