Application

Western Blotting Analysis: 1 µg/mL from a representative lot detected NG2 Chondroitin Sulfate Proteoglycan in Rat E16 brain tissuel lysate.

Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected NG2 Chondroitin Sulfate Proteoglycan in rat brain frozen sections, post-fixed in ethanol.

Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38, Cat. No. MAB5384-I, is a highly specific mouse monoclonal antibody that targets Chondrin Sulfate Protoglycan 4 and has been tested for use in Immunofluorescence and Western Blotting.

Research CategoryNeuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Chondroitin sulfate proteoglycan 4 (UniProt: Q00657; also known as Chondroitin sulfate proteoglycan NG2, HSN tumor-specific antigen) is encoded by the Cspg4 (also known as Ng2) gene (Gene ID: 81651) in rat. Chondroitin sulfate proteoglycan NG2 is a single-pass type I membrane protein that plays a role in cell proliferation and migration and stimulates endothelial cells motility during microvascular morphogenesis. It is present in neural cells and in extraneural tissues, especially in the developing mesenchyme. Its level of expression is highest on immature, proliferating cells and decreases when these cells begin to differentiate. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells. It is a major chondroitin sulfate proteoglycan that is produced after spinal cord injury and is expressed by macrophages and oligodendrocyte progenitors. It is also reported to inhibit neurite outgrowth and growth cone collapse during axon regeneration. NG2 chondroitin sulfate proteoglycan is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off in the mature form. (Ref.: Jones, LL et al. (2002). J. Neurosci. 22(7):2792-2803).

Immunogen

HEK293 cells expressing a truncated integral membrane form of NG2 consisting of amino acids 1592-2222.

Linkage

Replaces: MAB5384 and 05-710

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing PBS, pH 7.2, containing 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected NG2 Chondroitin Sulfate Proteoglycan in NIH/3T3 cell lysate.

Specificity

Clone 132.38 is a mouse monoclonal antibody that detects Chondroitin sulfate proteoglycan 4 in rat brain tissue.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~260 kDa observed. Uncharacterized bands may be observed in some lysate(s).