Analysis Note

Negative ControlNormal mouse IgG

Positive ControlEtoposide-treated HL-60 cells

Application

Immunoblotting (2.5 µg/ml)r>Immunocytochemistry (5-20 µg/ml)

General description

Protein A purified mouse monoclonal antibody. Recognizes poly-ADP-ribose.

This Anti-PAR (Ab-1) Mouse mAb (10H) is validated for use in Immunoblotting, Immunocytochemistry for the detection of PAR (Ab-1).

Recognizes poly(ADP)-ribose (PAR) in etopside-treated HL-60 cells.

Immunogen

purified poly(ADP-ribose)

Bovine

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kawamitsu, H., et al. 1984. Biochemistry23, 3771.

PAR can be induced in HL-60 cells by treatment with 67 µM etoposide for 3.5 h. Antibody should be titrated for optimal results in an individual system.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM NaCl, 50 mM HEPES, 1% BSA, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)