Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Pericentrin in HeLa cells.

This Anti-Pericentrin Antibody is validated for use in WB, IC for the detection of Pericentrin.

General description

Pericentrin binds to calmodulin and is expressed in the centrosome that serves as a multifunctional scaffold for anchoring proteins and protein complexes. Evidence suggests that pericentrin plays a role in microtubule nucleation and spindle organization. Recent studies show pericentrin plays a role in Majewski/micorcephalic osteodysplastic primordial dwarfism type II (MOPDII) and Seckel Syndrome. Recent use in network modeling indicates a direct link between breast cancer susceptibility to centrosome defects. Pericentrin is a potential marker for centrosome defects in cancer and cancer progression, as centrosomal abonormalities in solid tumors are accompanied by increased pericentrin levels and defects in pericentrin organization.

Immunogen

KLH-conjugated linear peptide corresponding to human Pericentrin.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Pericentrin in 10 µg of HeLa cell lysate. Western Blot was performed without Tween.

Specificity

Other homologies: Mouse (89% sequence homology). No homology to Rat was found.

Target description

~250 kDa observed.
Uniprot describes 2 isoforms produced by alternative splicing with molecular weights at 378 & 355 kDa However in endogenous lysates, Pericentrin is observed at ~250 kDa & ~150 kDa for western blots (Wen, Y., et al. (2008). J Biol Chem. 283(5):2793-2803).