Analysis Note

ControlHeLa cell lysate

Application

This Anti-Fibroblast-specific Protein 1 (S100A4) Antibody is validated for use in WB, IC for the detection of Fibroblast-specific Protein 1 (S100A4).

Research Sub CategoryIon & Transport ChannelsInflammation & Autoimmune Mechanisms

Research CategoryStem Cell ResearchInflammation & Immunology

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected S100A4 in mouse neural stem cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

S100A4, also known as Fibroblast-specific Protein 1, is a member of the Ca2+- dependent S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 is a metastasis factor that may function in motility, invasion, and tubulin polymerization. Increased expression of S100A4 has been associated with various types of cancer.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human S100A4.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected S100A4 in 10 µg of HeLa cell lysate.

Specificity

This antibody recognizes S100A4 at the C-terminus.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~12 kDa observed