ANTI-FLAG® M2 Affinity Gel purified immunoglobulin, buffered aqueous glycerol solution. Anti-FLAG M2 Affinity gel is a mouse monoclonal antibody that is covalently attached to agarose. The antibody binds FLAG at the N-terminal, Met-N-terminal, C-terminal and internal locations of fusion proteins. Binding is calcium-independent. Suspension in buffered saline containing azide as preservative and 50% glycerol. Anti-FLAG® M2 affinity gel has been used for western blotting, immunoprecipitation and for the purification of FLAG fusion proteins.
- Synonym: ANTI-FLAG® M2 Affinity Agarose Gel, Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG® M2 antibody produced in mouse
- Antibody Form: Purified immunoglobulin
- Antibody Product Type: Primary antibodies
- Clone: M2, monoclonal
- Form: Buffered aqueous glycerol solution
- Application(s): Affinity chromatography: suitable (FLAG® peptide, Glycine, pH3.5, 3x FLAG® peptide), Immunoprecipitation (IP): suitable
- Matrix: (4% agarose bead; 45-165µm bead size)
- Isotype: IgG1
- Capacity: >0.6 mg/mL, resin binding capacity (FLAG-BAP)
- Conjugate: Agarose conjugate
- Analyte Suitability: Proteins (FLAG® tag, 3x FLAG®, DYKDDDDK tag)
- Shipped In: Wet ice
- Storage Temp.: -20°C
This product has met the following criteria: