Application

Protein A-Sepharose was used:for immunoprecipitated placentas for protein and RNA analyses using Western blot technique.in chromatin immunoprecipitation to understand the link between Mediator and remodelling of chromatin structure at specific promoters.as lysis buffer to remove endogenous G-type Igs in protein extraction of brain samples from female rats.in immunoprecipitation of [³²P] phosphomyristin C of rabbit antiserum, to study the down-regulation of protein kinase C (PKC) and phosphomyristin C (PMC) during T-cell development.

Protein A-Sepharose^® is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose^® has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.

General description

Protein A-Sepharose when linked with antibody, helps improving antigen binding capacity of IgG crosslinked to column matrices. In this case, binding happens between Protein A and Fc portion of the IgG molecule leaving antigen specific sites free.

Legal Information

Sepharose is a registered trademark of Cytiva

Packaging

1, 1.5, 5 g in glass bottle

250 mg in glass bottle

Physical form

Supplied as lyophilized powder stabilized with lactose and dextran.

Quantity

Swelling: 1 g swells to approx. 4 ml.