Ni Sepharose excel is a his-tagged protein purification resin for minimized Ni-leakage and maximised protein recovery when samples contain stripping agents.
- Versatile – suitable for eukaryotic samples, including insect and CHO cells, as well as bacterial samples such as E. coli
- Direct sample load – of eukaryotic cell culture supernatants, eliminating the need for pretreatment of samples that usually cause nickel stripping
- Retained binding capacity of up to 10mg/mL resin
- Simplified sample handling – fewer steps needed compared with conventional workflow
- Flexible formats – prepacked and loose resin formats for optimised screening and preparative his-tag purification
Ni Sepharose excel is an IMAC resin precharged with nickel ions that are very strongly bound to a chelating ligand. Nickel ions remain bound to the ligand even after 24h incubation in 10mM EDTA.
Because of this strong binding, samples that typically cause stripping of metal ions can be loaded directly onto the resin without time-consuming pretreatment. Therefore, this his tag purification resin is especially suitable for purifying histidine-tagged (his-tagged) proteins secreted into eukaryotic cell culture supernatants.
The flow properties of Ni Sepharose excel nickel resin make it well-suited to purification at a wide range of scales. This resin can handle loading of large sample volumes, so you can purify low concentrations of target proteins using manual or automated methods.
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