Glutathione Sepharose High Performance 100 ml

Code: 17527902 L1-23

Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed.

Fast, one-step GST-tag...

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Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed.

Fast, one-step GST-tagged protein purification.
Mild elution conditions preserve protein antigenicity and function.
Excellent scale-up due to high flow properties.

Resin for GST-tagged protein purification

Glutathione Sepharose 4 Fast Flow is used to purify glutathione S-transferase (GST)-tagged proteins from bacterial lysates. It is also suitable for purifying other S-transferases or glutathione-dependent proteins.

	Glutathione Sepharose 4 Fast Flow
Flow velocity, cm/h	<450
Ligand	Glutathione and 10-carbon linker arm
Particle size, d50V	~90µm
Matrix	Highly cross-linked 4% agarose
Ligand Coupling Method	Epoxy activation
Binding Capacity/ml Chromatography Medium	>10mg/mL
pH stability, operational	3 - 12
pH stability, CIP	3 - 12
Pressure/Flow Specification	base matrix 150-250cm/h, 100kPa, XK 50/60 column, bed height 25cm
Storage	4 to 30°C, 20% Ethanol
Chemical stability	Commonly used aqueous buffers, e.g. 1M acetate, pH 6.0, 0.1M NaOH, 70% ethanol or 6M guanidine-hydrochloride for 1h at room temperature
Chromatography technique	GST-tagged protein purification