HiTrap IgY Purification HP

Code: 17511101 D2-84

HiTrap IgY Purification HP is a 5mL column prepacked with Sepharose High Performance for fast and easy purification of IgY from egg yolk.

  • Each column binds IgY from a one-quarter ...


    read more

    Your Price
    £736.00 EACH
    £883.20 inc. VAT

HiTrap IgY Purification HP is a 5mL column prepacked with Sepharose High Performance for fast and easy purification of IgY from egg yolk.

  • Each column binds IgY from a one-quarter portion of egg yolk (approx. 100mg IgY). For greater capacity, several columns may be connected in series.
  • Packed with a thiophilic adsorption resin, the ligand, 2-mercaptopyridine is coupled to Sepharose High Performance.
  • Recovery and purity of IgY are excellent.
  • Can be operated with a liquid chromatography system such as ÄKTA design.
  • The top and bottom frits are manufactured from porous polyethylene
  • Connectors for usage with different chromatography systems and other equipment are included

HiTrap IgY Purification HP is packed with a thiophilic adsorption resin, 2-mercaptopyridine coupled to Sepharose High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

HiTrap IgY Purification HP
Bed dimensions, mm16 x 25
Bed height, mm25
Flow rate, mL/min<20
ApplicationUsed for purification of IgY from egg yolk The lipids from an egg yolk were removed by precipitation with water and centrifugation at 4°C
Bed volume, mL5
Column i.d.16
Pressure max. (over the packed bed during operation)5 bar [0.5MPa] (70psi)
Storage+4 to 30°C. 20% Ethanol
Chromatography techniqueAntibody affinity chromatography
This product has met the following criteria: