Protein A Sepharose CL-4B 500 ml

Code: 17096302 L1-23

Protein A Sepharose CL-4B is a trusted, well-documented affinity resin for immunoprecipitation procedures. It can also be used for antibody purification.

  • High binding capacity of ...


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    £18,742.00 EACH
    £22,490.40 inc. VAT

Protein A Sepharose CL-4B is a trusted, well-documented affinity resin for immunoprecipitation procedures. It can also be used for antibody purification.

  • High binding capacity of 20mg human IgG/mL.
  • High selectivity and low nonspecific adsorption.
  • Well suited for immunoprecipitation procedures.

Use in immunoprecipitation procedures

Protein A Sepharose CL-4B resin offers high specificity and capacity, making it dependable for precise, reproducible immunoprecipitation procedures – whether the end goal is determining protein quality, analyzing protein interactions, or assessing post-translational modifications.

Protein A Sepharose CL-4B
Particle Size, um45 - 165
Flow velocity150cm/h at 25°C, HR 16/10 columns, 5cm bed height
LigandNative Protein A
Particle size, d50V~90µm
MatrixCross-linked agarose, 4%
Ligand Coupling MethodCyanogen bromide activation
Binding Capacity/ml Chromatography Medium~20mg human lgG/mL resin
Ligand density3mg Protein A/mL medium
pH stability, operational3 - 9
pH stability, CIP2 - 10
Storage2 to 8°C, 20% Ethanol
Chemical stabilityStable in commonly used aqueous buffers, 6M guanidine-HCl, 8M urea
Sanitization Conditions2% Hibitane/20% Ethanol or with 70% Ethanol
Chromatography techniqueAntibody affinity chromatography
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17078001
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17096303
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